GALLERY | labgroup3website

PHOTO & VIDEO GALLERY

Here is a collection of photos and videos of our experiments and days in the lab.

Photos

The micropipette we will use for various experiments in this lab	Our template DNA and primers being kept on ice
Weighing out water to balance the centrifuge	The collective vial is put into the centrifuge with a vial of water of equal mass opposite to balance
Our group's vial	Patrick bringing up our vial on ice to the thermal cycler
The thermal cycler machine will run the samples through the process of PCR	Putting our vial in the thermal cycler
The various group's vials in the thermal cycler	The results of the gel electrophoresis
All of the necessary materials for the yeast transformation	Sterile water for the yeast transformation
Salmon sperm for the yeast transformation	A beaker for the supernatant waste
Our vial where we mixed all the components for the yeast transformation	The vortex machine used to mix up the vials
The centrifuge machine used in the yeast transformation	The 96-well plate we would use for a practice serial dilution
Putting the 96-well plate into the plate reader (spectrophotometer)	The data from our practice spectrophotometer run
The graphed results of our practice run with the spectrophotometer	Practicing our sterile technique with ethanol burners
Pipetting our yeast onto agar plates	Pipetting our yeast onto agar plates close to ethanol burner to avoid contamination
The YPD media we made	Our YPD jar in the autoclave machine
Our liquid YPD tubes	The Fluorescence Activated Cell Sorting (FACS) machine
A description of each of our agar plates	First set of pictures of colonies for WT yeast in normal conditions
First set of pictures of colonies for mutant yeast in normal conditions	First set of pictures of colonies for WT yeast on caffeine agar
First set of pictures of colonies for mutant yeast on caffeine agar	First set of pictures of colonies for WT yeast on bleomycin agar
First set of pictures of colonies for mutant yeast on bleomycin agar	The 96-well plate with our YPD and yeast for growth curve analysis
Growth curves for WT strain	Growth curves from mutant strain
Our 3D model from Tinkercad	Our printed wax paper
Melting the wax paper to create a hydrophobic barrier	Covering the back of our wax paper with vaseline
Pipetting agar onto the white squares of our wax paper	Pipetting agar onto the white squares of our wax paper
The contamination on our first wax paper trial	Putting nail polish on the wax paper for second trial
Our sterile setup for the wax print experiment	The updated design of our 3D model
First successful trial of wax paper experiment	First successful trial of wax paper experiment
First successful trial of wax paper experiment	First successful trial of wax paper experiment
Wax paper experiment with optimal square only	Optimal square trial results
Optimal square trial results	Table of agar ratios used on different sized squares
Our model in the dried PDMS	3D print
2nd 3D print with structural supports	Covering our 3D model with PDMS
Our model covered in PDMS	Presenting our poster at the HHMI symposium
Suspending our model with the PDMS in NaOH	The NaOH used to melt away our model